Inventors:
Jonathan T. Skare - College Station TX
Ellen S. Shang - Calabasas CA
Cheryl I. Champion - Los Angeles CA
David R. Blanco - Calabassas CA
James N. Miller - Northridge CA
Michael A. Lovett - Los Angeles CA
Tajib A. Mirzabekov - Newton MA
Bruce L. Kagan - Pacific Palisades CA
Paul Tempst - New York NY
Denise M. Foley - Orange CA
Assignee:
The Regents of the University of California - Oakland CA
International Classification:
A61K 3900
A61K 3902
G01N 33554
C07K 100
Abstract:
The present invention presents three B. burgdorferi membrane proteins: Oms28, Oms45, and Oms66, each of about 28, 45, and 66 kDa respectively; and with average single channel conductances of about 0. 6, 0. 22, and 9. 7 nS, respectively. Also disclosed are the methods for purifying these proteins from B. burgdorferi, methods for producing antibodies to these proteins, and the resulting antibodies. These proteins and their immunogenic fragments, and antibodies capable of binding to them are useful for inducing an immune response to pathogenic B. burgdorferi as well as providing a diagnostic target for Lyme disease. Further disclosed are the nucleotide and amino acid sequences, the cloning of the genes encoding the proteins and their recombinant proteins, and methods for obtaining the foregoing. Other B. burgdorferi outer membrane spanning proteins (Oms) obtainable by the isolation and purification methods of the present invention.